SrasV1, Main, Exploration, bibRecord, 000F27

The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles

Identifieur interne : 000F27 ( Main/Exploration ); précédent : 000F26; suivant : 000F28

The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles

Auteurs : Makoto Ujike ; Cheng Huang ; Kazuya Shirato ; Shinji Makino ; Fumihiro Taguchi

Source :

The Journal of General Virology [ 0022-1317 ] ; 2016.

RBID : PMC:5764123

Descripteurs français

KwdFr :
- Animaux, Appareil de Golgi (), Assemblage viral, Glycoprotéine de spicule des coronavirus (génétique), Glycoprotéine de spicule des coronavirus (métabolisme), Humains, Lignée cellulaire, Protéines de la matrice virale (métabolisme), Protéines mutantes (génétique), Protéines mutantes (métabolisme), Signaux de triage des protéines, Transport de protéines, Virosomes (métabolisme), Virus du SRAS (physiologie).
MESH :
- génétique : Glycoprotéine de spicule des coronavirus, Protéines mutantes.
- métabolisme : Glycoprotéine de spicule des coronavirus, Protéines de la matrice virale, Protéines mutantes, Virosomes.
- physiologie : Virus du SRAS.
- Animaux, Appareil de Golgi, Assemblage viral, Humains, Lignée cellulaire, Signaux de triage des protéines, Transport de protéines.

English descriptors

KwdEn :
- Animals, Cell Line, Golgi Apparatus (chemistry), Humans, Mutant Proteins (genetics), Mutant Proteins (metabolism), Protein Sorting Signals, Protein Transport, SARS Virus (physiology), Spike Glycoprotein, Coronavirus (genetics), Spike Glycoprotein, Coronavirus (metabolism), Viral Matrix Proteins (metabolism), Virosomes (metabolism), Virus Assembly.
MESH :
- chemical , genetics : Mutant Proteins, Spike Glycoprotein, Coronavirus.
- chemistry : Golgi Apparatus.
- chemical , metabolism : Mutant Proteins, Spike Glycoprotein, Coronavirus, Viral Matrix Proteins, Virosomes.
- physiology : SARS Virus.
- Animals, Cell Line, Humans, Protein Sorting Signals, Protein Transport, Virus Assembly.

Abstract

The cytoplasmic tails of some coronavirus (CoV) spike (S) proteins contain an endoplasmic reticulum retrieval signal (ERRS) that can retrieve S proteins from the Golgi to the endoplasmic reticulum (ER); this process is thought to accumulate S proteins at the CoV budding site, the ER-Golgi intermediate compartment (ERGIC), and to facilitate S protein incorporation into virions. However, we showed previously that porcine epidemic diarrhoea CoV S proteins lacking the ERRS were efficiently incorporated into virions, similar to the original virus. Thus, the precise role of the ERRS in virus assembly remains unclear. Here, the roles of the S protein ERRS in severe acute respiratory syndrome CoV (SARS-CoV) intracellular trafficking and S incorporation into virus-like particles (VLPs) are described. Intracellular trafficking and indirect immunofluorescence analysis suggested that when M protein was present, wild-type S protein (wtS) could be retained in the pre- and post-medial Golgi compartments intracellularly and co-localized with M protein in the Golgi. In contrast, mutant S protein lacking the ERRS was distributed throughout the ER and only partially co-localized with M protein. Moreover, the intracellular accumulation of mutant S protein, particularly at the post-medial Golgi compartment, was significantly reduced compared with wtS. A VLP assay suggested that wtS that reached the post-medial compartment could be returned to the ERGIC for subsequent incorporation into VLPs, while mutant S protein could not. These results suggest that the ERRS of SARS-CoV contributes to intracellular S protein accumulation specifically in the post-medial Golgi compartment and to S protein incorporation into VLPs.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5764123

DOI: 10.1099/jgv.0.000494
PubMed: 27145752
PubMed Central: 5764123

Affiliations:

Le document en format XML

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<term>Mutant Proteins (metabolism)</term>
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<term>Glycoprotéine de spicule des coronavirus (métabolisme)</term>
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<front><div type="abstract" xml:lang="en"><p>The cytoplasmic tails of some coronavirus (CoV) spike (S) proteins contain an endoplasmic reticulum retrieval signal (ERRS) that can retrieve S proteins from the Golgi to the endoplasmic reticulum (ER); this process is thought to accumulate S proteins at the CoV budding site, the ER-Golgi intermediate compartment (ERGIC), and to facilitate S protein incorporation into virions. However, we showed previously that porcine epidemic diarrhoea CoV S proteins lacking the ERRS were efficiently incorporated into virions, similar to the original virus. Thus, the precise role of the ERRS in virus assembly remains unclear. Here, the roles of the S protein ERRS in severe acute respiratory syndrome CoV (SARS-CoV) intracellular trafficking and S incorporation into virus-like particles (VLPs) are described. Intracellular trafficking and indirect immunofluorescence analysis suggested that when M protein was present, wild-type S protein (wtS) could be retained in the pre- and post-medial Golgi compartments intracellularly and co-localized with M protein in the Golgi. In contrast, mutant S protein lacking the ERRS was distributed throughout the ER and only partially co-localized with M protein. Moreover, the intracellular accumulation of mutant S protein, particularly at the post-medial Golgi compartment, was significantly reduced compared with wtS. A VLP assay suggested that wtS that reached the post-medial compartment could be returned to the ERGIC for subsequent incorporation into VLPs, while mutant S protein could not. These results suggest that the ERRS of SARS-CoV contributes to intracellular S protein accumulation specifically in the post-medial Golgi compartment and to S protein incorporation into VLPs.</p>
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The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles

The contribution of the cytoplasmic retrieval signal of severe acute respiratory syndrome coronavirus to intracellular accumulation of S proteins and incorporation of S protein into virus-like particles

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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